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KMID : 1041219990410020108
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Journal of Sericultural and Entomological Science
1999 Volume.41 No. 2 p.108 ~ p.115
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Improved Method for the Preparation of Silk Fibroin Hydrolysates
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Madyarov, Shukhrat
Lee, Kwang Gill/Yeo, Joo Hong/Nam, Jin/Lee, Yong Woo
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Abstract
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An improvement of methods in fibroin hydrolysates preparation significantly enlarges their applications to practical use. Acidic hydrolysis by hydrochloric acid is one of the methods for silk fibroin depolymerization. A low yield of final product and long time of the process are the demerits of this method. Possibility of preparation of water-soluble silk hydrolysates with more yield and less expenses is investigated in this study. Such possibility is occurred with the increasing treatment temperature and simultaneously decreasing treatment time, concentration of hydrochloric acid and respectively, the concentration of sodium hydroxide used for neutralization of hydrolysates after hydrolysis. Colour is decreased in this case and a small amount of activated carbon is demanded for decoluorization of hydrolysates. Production cost of desalted products are reduced, too. Protection of hydrolysates against precipitation after neutralization, and separation and during concentrating process is the other merit of this method. Creamy-coloured insoluble silk powder is the remainder of hydrolyzed fibroin. This is the only defect of the proposed method. However, this insoluble powder can be used as a matrix for the irnmobilization of enzymes and other physiological active substances. Fine particles of this powder can be used as additives for artificial diets and cultural media, as well as raw materials for polymer membranes, etc.
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